Review



pet 21 b  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc pet 21 b
    Pet 21 B, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+21+b/pIC247+(Plasmid+%2369741)/pmc12281481-0-4-6
    Average 93 stars, based on 5 article reviews
    pet 21 b - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: High-Throughput Screening Tool to Identify Small Molecule Inhibitors of Telomerase
    Article Snippet: .. TcTERT expression plasmid , pET-21 b(+) (Addgene# 69741-3). .. BBT-dGTP, BBT-GTP , Jena Bioscience.

    Article Title: Mutations in the coordination spheres of T1 Cu affect Cu 2+ -activation of the laccase from Thermus thermophilus.
    Article Snippet: .. E. coli DH5a and E. coli OrigamiTM 2 (DE3) (Merck Millipore, Burlington, USA) were used for plasmid amplification and heterologous recombinant LAC protein expression, respectively. pET-21 b (Addgene, Cambridge, USA) was used for gene expression of Tt-LAC WT and mutants. .. E. coli strains were grown in LB media (Merck, Darmstadt, Germany) with selectable antibiotics.

    Plasmid Preparation:

    Article Title: High-Throughput Screening Tool to Identify Small Molecule Inhibitors of Telomerase
    Article Snippet: .. TcTERT expression plasmid , pET-21 b(+) (Addgene# 69741-3). .. BBT-dGTP, BBT-GTP , Jena Bioscience.

    Article Title: Mutations in the coordination spheres of T1 Cu affect Cu 2+ -activation of the laccase from Thermus thermophilus.
    Article Snippet: .. E. coli DH5a and E. coli OrigamiTM 2 (DE3) (Merck Millipore, Burlington, USA) were used for plasmid amplification and heterologous recombinant LAC protein expression, respectively. pET-21 b (Addgene, Cambridge, USA) was used for gene expression of Tt-LAC WT and mutants. .. E. coli strains were grown in LB media (Merck, Darmstadt, Germany) with selectable antibiotics.

    Amplification:

    Article Title: Mutations in the coordination spheres of T1 Cu affect Cu 2+ -activation of the laccase from Thermus thermophilus.
    Article Snippet: .. E. coli DH5a and E. coli OrigamiTM 2 (DE3) (Merck Millipore, Burlington, USA) were used for plasmid amplification and heterologous recombinant LAC protein expression, respectively. pET-21 b (Addgene, Cambridge, USA) was used for gene expression of Tt-LAC WT and mutants. .. E. coli strains were grown in LB media (Merck, Darmstadt, Germany) with selectable antibiotics.

    Recombinant:

    Article Title: Mutations in the coordination spheres of T1 Cu affect Cu 2+ -activation of the laccase from Thermus thermophilus.
    Article Snippet: .. E. coli DH5a and E. coli OrigamiTM 2 (DE3) (Merck Millipore, Burlington, USA) were used for plasmid amplification and heterologous recombinant LAC protein expression, respectively. pET-21 b (Addgene, Cambridge, USA) was used for gene expression of Tt-LAC WT and mutants. .. E. coli strains were grown in LB media (Merck, Darmstadt, Germany) with selectable antibiotics.

    Gene Expression:

    Article Title: Mutations in the coordination spheres of T1 Cu affect Cu 2+ -activation of the laccase from Thermus thermophilus.
    Article Snippet: .. E. coli DH5a and E. coli OrigamiTM 2 (DE3) (Merck Millipore, Burlington, USA) were used for plasmid amplification and heterologous recombinant LAC protein expression, respectively. pET-21 b (Addgene, Cambridge, USA) was used for gene expression of Tt-LAC WT and mutants. .. E. coli strains were grown in LB media (Merck, Darmstadt, Germany) with selectable antibiotics.



    Similar Products

    pet 21 b  (Addgene inc)
    93
    Addgene inc pet 21 b
    Pet 21 B, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+21+b/pIC247+(Plasmid+%2369741)/pmc12281481-0-4-6
    Average 93 stars, based on 1 article reviews
    pet 21 b - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    pet-21 b (+) vector  (Millipore)
    90
    Millipore pet-21 b (+) vector
    Pet 21 B (+) Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+21+b/pet28a/us11807880-273-17-21
    Average 90 stars, based on 1 article reviews
    pet-21 b (+) vector - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    pet 21 b (+) vector  (Millipore)
    90
    Millipore pet 21 b (+) vector
    Pet 21 B (+) Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+21+b/pet28a/pmc10589169-71-6-11
    Average 90 stars, based on 1 article reviews
    pet 21 b (+) vector - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    pet-21(b) expression vector  (Millipore)
    90
    Millipore pet-21(b) expression vector
    All data are for cultures of E. coli strain BL21(DE3) ΔiscR , and both iron and cysteine were included in the growth medium. (Fig. 1A) Optical density at 600 nm (shown on a logarithmic scale) of cultures during aerobic (orange circles) and anaerobic (blue circles) growth phases for cells containing the pACYCDuet-1– hydGX – hydEF and <t>pET-21(b)</t> shydA1*–Strep-tag II plasmids. The pH of culture media (×) was also measured. Data for cultures with cells containing the pET-21(b) shydA–Strep-tag II plasmid instead of pET-21(b) shydA1*–Strep-tag II were similar and are not shown. (Fig. 1B) Cell lysate-based hydrogenase activities (µmol MV reduced·min −1 ·mg −1 total protein) for active CpI (red squares) and HydA1 hydrogenase (green triangles) were determined using the methyl viologen reduction assay. Data are the average for n = 3 cultures examined ± standard deviations. (Fig. 1C) SDS-PAGE analysis for the soluble fractions of final cell lysates after the anoxic co-expression of HydA1 or CpI and the HydE, HydF, and HydG maturases: (Lane 1) the molecular weight markers are from the Mark12 TM protein ladder (Invitrogen); (Lane 2) soluble cell lysate protein content for E. coli strain BL21(DE3) ΔiscR following expression of no heterologous proteins from recombinant DNA plasmids; (Lane 3) co-expression of only the HydE, HydF, and HydG maturases; (Lane 4) co-expression of HydE, HydF, HydG, and HydA1– Strep -tag II; and (Lane 5) co-expression of HydE, HydF, HydG, and CpI– Strep -tag II.
    Pet 21(b) Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+21+b/pet28a/pmc02991362-143-7-10
    Average 90 stars, based on 1 article reviews
    pet-21(b) expression vector - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    pet-21(b) hydf–strep -tag ii  (Millipore)
    90
    Millipore pet-21(b) hydf–strep -tag ii
    All data are for cultures of E. coli strain BL21(DE3) ΔiscR , and both iron and cysteine were included in the growth medium. (Fig. 1A) Optical density at 600 nm (shown on a logarithmic scale) of cultures during aerobic (orange circles) and anaerobic (blue circles) growth phases for cells containing the pACYCDuet-1– hydGX – hydEF and <t>pET-21(b)</t> shydA1*–Strep-tag II plasmids. The pH of culture media (×) was also measured. Data for cultures with cells containing the pET-21(b) shydA–Strep-tag II plasmid instead of pET-21(b) shydA1*–Strep-tag II were similar and are not shown. (Fig. 1B) Cell lysate-based hydrogenase activities (µmol MV reduced·min −1 ·mg −1 total protein) for active CpI (red squares) and HydA1 hydrogenase (green triangles) were determined using the methyl viologen reduction assay. Data are the average for n = 3 cultures examined ± standard deviations. (Fig. 1C) SDS-PAGE analysis for the soluble fractions of final cell lysates after the anoxic co-expression of HydA1 or CpI and the HydE, HydF, and HydG maturases: (Lane 1) the molecular weight markers are from the Mark12 TM protein ladder (Invitrogen); (Lane 2) soluble cell lysate protein content for E. coli strain BL21(DE3) ΔiscR following expression of no heterologous proteins from recombinant DNA plasmids; (Lane 3) co-expression of only the HydE, HydF, and HydG maturases; (Lane 4) co-expression of HydE, HydF, HydG, and HydA1– Strep -tag II; and (Lane 5) co-expression of HydE, HydF, HydG, and CpI– Strep -tag II.
    Pet 21(b) Hydf–Strep Tag Ii, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+21+b/pet+21++++t7+promoter+lac+operator++laciq/pmc03105041-106-32-10
    Average 90 stars, based on 1 article reviews
    pet-21(b) hydf–strep -tag ii - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    pet-21(b) hydf  (Millipore)
    90
    Millipore pet-21(b) hydf
    All data are for cultures of E. coli strain BL21(DE3) ΔiscR , and both iron and cysteine were included in the growth medium. (Fig. 1A) Optical density at 600 nm (shown on a logarithmic scale) of cultures during aerobic (orange circles) and anaerobic (blue circles) growth phases for cells containing the pACYCDuet-1– hydGX – hydEF and <t>pET-21(b)</t> shydA1*–Strep-tag II plasmids. The pH of culture media (×) was also measured. Data for cultures with cells containing the pET-21(b) shydA–Strep-tag II plasmid instead of pET-21(b) shydA1*–Strep-tag II were similar and are not shown. (Fig. 1B) Cell lysate-based hydrogenase activities (µmol MV reduced·min −1 ·mg −1 total protein) for active CpI (red squares) and HydA1 hydrogenase (green triangles) were determined using the methyl viologen reduction assay. Data are the average for n = 3 cultures examined ± standard deviations. (Fig. 1C) SDS-PAGE analysis for the soluble fractions of final cell lysates after the anoxic co-expression of HydA1 or CpI and the HydE, HydF, and HydG maturases: (Lane 1) the molecular weight markers are from the Mark12 TM protein ladder (Invitrogen); (Lane 2) soluble cell lysate protein content for E. coli strain BL21(DE3) ΔiscR following expression of no heterologous proteins from recombinant DNA plasmids; (Lane 3) co-expression of only the HydE, HydF, and HydG maturases; (Lane 4) co-expression of HydE, HydF, HydG, and HydA1– Strep -tag II; and (Lane 5) co-expression of HydE, HydF, HydG, and CpI– Strep -tag II.
    Pet 21(b) Hydf, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+21+b/pet+21++++t7+promoter+lac+operator++laciq/pmc03105041-106-29-10
    Average 90 stars, based on 1 article reviews
    pet-21(b) hydf - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    pet-21(b) hyde–strep -tag ii  (Millipore)
    90
    Millipore pet-21(b) hyde–strep -tag ii
    All data are for cultures of E. coli strain BL21(DE3) ΔiscR , and both iron and cysteine were included in the growth medium. (Fig. 1A) Optical density at 600 nm (shown on a logarithmic scale) of cultures during aerobic (orange circles) and anaerobic (blue circles) growth phases for cells containing the pACYCDuet-1– hydGX – hydEF and <t>pET-21(b)</t> shydA1*–Strep-tag II plasmids. The pH of culture media (×) was also measured. Data for cultures with cells containing the pET-21(b) shydA–Strep-tag II plasmid instead of pET-21(b) shydA1*–Strep-tag II were similar and are not shown. (Fig. 1B) Cell lysate-based hydrogenase activities (µmol MV reduced·min −1 ·mg −1 total protein) for active CpI (red squares) and HydA1 hydrogenase (green triangles) were determined using the methyl viologen reduction assay. Data are the average for n = 3 cultures examined ± standard deviations. (Fig. 1C) SDS-PAGE analysis for the soluble fractions of final cell lysates after the anoxic co-expression of HydA1 or CpI and the HydE, HydF, and HydG maturases: (Lane 1) the molecular weight markers are from the Mark12 TM protein ladder (Invitrogen); (Lane 2) soluble cell lysate protein content for E. coli strain BL21(DE3) ΔiscR following expression of no heterologous proteins from recombinant DNA plasmids; (Lane 3) co-expression of only the HydE, HydF, and HydG maturases; (Lane 4) co-expression of HydE, HydF, HydG, and HydA1– Strep -tag II; and (Lane 5) co-expression of HydE, HydF, HydG, and CpI– Strep -tag II.
    Pet 21(b) Hyde–Strep Tag Ii, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+21+b/pet+21++++t7+promoter+lac+operator++laciq/pmc03105041-106-25-10
    Average 90 stars, based on 1 article reviews
    pet-21(b) hyde–strep -tag ii - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    pet-21(b) strep -tag ii– hydg  (Millipore)
    90
    Millipore pet-21(b) strep -tag ii– hydg
    All data are for cultures of E. coli strain BL21(DE3) ΔiscR , and both iron and cysteine were included in the growth medium. (Fig. 1A) Optical density at 600 nm (shown on a logarithmic scale) of cultures during aerobic (orange circles) and anaerobic (blue circles) growth phases for cells containing the pACYCDuet-1– hydGX – hydEF and <t>pET-21(b)</t> shydA1*–Strep-tag II plasmids. The pH of culture media (×) was also measured. Data for cultures with cells containing the pET-21(b) shydA–Strep-tag II plasmid instead of pET-21(b) shydA1*–Strep-tag II were similar and are not shown. (Fig. 1B) Cell lysate-based hydrogenase activities (µmol MV reduced·min −1 ·mg −1 total protein) for active CpI (red squares) and HydA1 hydrogenase (green triangles) were determined using the methyl viologen reduction assay. Data are the average for n = 3 cultures examined ± standard deviations. (Fig. 1C) SDS-PAGE analysis for the soluble fractions of final cell lysates after the anoxic co-expression of HydA1 or CpI and the HydE, HydF, and HydG maturases: (Lane 1) the molecular weight markers are from the Mark12 TM protein ladder (Invitrogen); (Lane 2) soluble cell lysate protein content for E. coli strain BL21(DE3) ΔiscR following expression of no heterologous proteins from recombinant DNA plasmids; (Lane 3) co-expression of only the HydE, HydF, and HydG maturases; (Lane 4) co-expression of HydE, HydF, HydG, and HydA1– Strep -tag II; and (Lane 5) co-expression of HydE, HydF, HydG, and CpI– Strep -tag II.
    Pet 21(b) Strep Tag Ii– Hydg, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+21+b/pet+21++++t7+promoter+lac+operator++laciq/pmc03105041-106-39-10
    Average 90 stars, based on 1 article reviews
    pet-21(b) strep -tag ii– hydg - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    pet-21(b) hydg–strep -tag ii  (Millipore)
    90
    Millipore pet-21(b) hydg–strep -tag ii
    All data are for cultures of E. coli strain BL21(DE3) ΔiscR , and both iron and cysteine were included in the growth medium. (Fig. 1A) Optical density at 600 nm (shown on a logarithmic scale) of cultures during aerobic (orange circles) and anaerobic (blue circles) growth phases for cells containing the pACYCDuet-1– hydGX – hydEF and <t>pET-21(b)</t> shydA1*–Strep-tag II plasmids. The pH of culture media (×) was also measured. Data for cultures with cells containing the pET-21(b) shydA–Strep-tag II plasmid instead of pET-21(b) shydA1*–Strep-tag II were similar and are not shown. (Fig. 1B) Cell lysate-based hydrogenase activities (µmol MV reduced·min −1 ·mg −1 total protein) for active CpI (red squares) and HydA1 hydrogenase (green triangles) were determined using the methyl viologen reduction assay. Data are the average for n = 3 cultures examined ± standard deviations. (Fig. 1C) SDS-PAGE analysis for the soluble fractions of final cell lysates after the anoxic co-expression of HydA1 or CpI and the HydE, HydF, and HydG maturases: (Lane 1) the molecular weight markers are from the Mark12 TM protein ladder (Invitrogen); (Lane 2) soluble cell lysate protein content for E. coli strain BL21(DE3) ΔiscR following expression of no heterologous proteins from recombinant DNA plasmids; (Lane 3) co-expression of only the HydE, HydF, and HydG maturases; (Lane 4) co-expression of HydE, HydF, HydG, and HydA1– Strep -tag II; and (Lane 5) co-expression of HydE, HydF, HydG, and CpI– Strep -tag II.
    Pet 21(b) Hydg–Strep Tag Ii, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+21+b/pet+21++++t7+promoter+lac+operator++laciq/pmc03105041-106-45-15
    Average 90 stars, based on 1 article reviews
    pet-21(b) hydg–strep -tag ii - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    All data are for cultures of E. coli strain BL21(DE3) ΔiscR , and both iron and cysteine were included in the growth medium. (Fig. 1A) Optical density at 600 nm (shown on a logarithmic scale) of cultures during aerobic (orange circles) and anaerobic (blue circles) growth phases for cells containing the pACYCDuet-1– hydGX – hydEF and pET-21(b) shydA1*–Strep-tag II plasmids. The pH of culture media (×) was also measured. Data for cultures with cells containing the pET-21(b) shydA–Strep-tag II plasmid instead of pET-21(b) shydA1*–Strep-tag II were similar and are not shown. (Fig. 1B) Cell lysate-based hydrogenase activities (µmol MV reduced·min −1 ·mg −1 total protein) for active CpI (red squares) and HydA1 hydrogenase (green triangles) were determined using the methyl viologen reduction assay. Data are the average for n = 3 cultures examined ± standard deviations. (Fig. 1C) SDS-PAGE analysis for the soluble fractions of final cell lysates after the anoxic co-expression of HydA1 or CpI and the HydE, HydF, and HydG maturases: (Lane 1) the molecular weight markers are from the Mark12 TM protein ladder (Invitrogen); (Lane 2) soluble cell lysate protein content for E. coli strain BL21(DE3) ΔiscR following expression of no heterologous proteins from recombinant DNA plasmids; (Lane 3) co-expression of only the HydE, HydF, and HydG maturases; (Lane 4) co-expression of HydE, HydF, HydG, and HydA1– Strep -tag II; and (Lane 5) co-expression of HydE, HydF, HydG, and CpI– Strep -tag II.

    Journal: PLoS ONE

    Article Title: High-Yield Expression of Heterologous [FeFe] Hydrogenases in Escherichia coli

    doi: 10.1371/journal.pone.0015491

    Figure Lengend Snippet: All data are for cultures of E. coli strain BL21(DE3) ΔiscR , and both iron and cysteine were included in the growth medium. (Fig. 1A) Optical density at 600 nm (shown on a logarithmic scale) of cultures during aerobic (orange circles) and anaerobic (blue circles) growth phases for cells containing the pACYCDuet-1– hydGX – hydEF and pET-21(b) shydA1*–Strep-tag II plasmids. The pH of culture media (×) was also measured. Data for cultures with cells containing the pET-21(b) shydA–Strep-tag II plasmid instead of pET-21(b) shydA1*–Strep-tag II were similar and are not shown. (Fig. 1B) Cell lysate-based hydrogenase activities (µmol MV reduced·min −1 ·mg −1 total protein) for active CpI (red squares) and HydA1 hydrogenase (green triangles) were determined using the methyl viologen reduction assay. Data are the average for n = 3 cultures examined ± standard deviations. (Fig. 1C) SDS-PAGE analysis for the soluble fractions of final cell lysates after the anoxic co-expression of HydA1 or CpI and the HydE, HydF, and HydG maturases: (Lane 1) the molecular weight markers are from the Mark12 TM protein ladder (Invitrogen); (Lane 2) soluble cell lysate protein content for E. coli strain BL21(DE3) ΔiscR following expression of no heterologous proteins from recombinant DNA plasmids; (Lane 3) co-expression of only the HydE, HydF, and HydG maturases; (Lane 4) co-expression of HydE, HydF, HydG, and HydA1– Strep -tag II; and (Lane 5) co-expression of HydE, HydF, HydG, and CpI– Strep -tag II.

    Article Snippet: PCR products were then cloned into the pET-21(b) expression vector (Novagen).

    Techniques: Strep-tag, Plasmid Preparation, SDS Page, Expressing, Molecular Weight, Recombinant